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Nanopore support #14

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arunvv90 opened this issue May 14, 2021 · 3 comments
Open

Nanopore support #14

arunvv90 opened this issue May 14, 2021 · 3 comments
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@arunvv90
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I have run the Falco using the following commands
falco --nano reads.fastq.gz
I am using nanopore reads. Html report says it is using the sanger/illumina encoding even though I have specified the nanopore. Do I need to change anything else. Publication says falco has been tested with both Illumina and nanopore data. Does falco supports the following for nanopore reads
-Q score of nanopore instead of Illumina phread score

  • Does it detects the adapter or barcodes of nanopore reads
  • Does it supports the overrepresentation feature for nanopore reads

summary.txt
fastqc_data.txt

@Hedi65
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Hedi65 commented Sep 3, 2021

i have the same problem

@guilhermesena1
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Hi,

The "Sanger/Illumina" is currently hard-coded into the report, I am working on the next release to address this issue and print the correct encoding when --nanopore option is set. That said, all the modules should work the same regardless if reads are from Illumina or nanopore. Specifically, per-base sequence content, adapters, quality distribution, overrepresented sequences etc. can still be interpreted the same way even if the encoding says Illumina. I apologize sincerely for the confusion!

@guilhermesena1 guilhermesena1 added the bug Something isn't working label Sep 9, 2021
@andrewdavidsmith
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@guilhermesena1 @arunvv90 I'm wondering if this issue is about the html, the documentation, or something wrong in the function of falco? @Hedi65 Can you clarify which same problem? Is it lack of information, uncertainties, the wrong name in the HMTL output?

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