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POLCA fails to generate .vcf file #317
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Hi, I have encountered a similar problem when running POLCA (MaSuRCA v4.1.0) on a Flye assembly in a second round of polishing. There probably is a better to way to fix this, but as a workaround I have changed that line to: I guess there might be a similar problem in your case in that chained command for generating the final .vcf file. |
Thanks for the reply, modifying that line as you suggested worked for me in v4.0.9 |
I had the same issue, corrected line 178, and it works perfectly in v4.1.0. |
Hi there,
I'm running Masurca v4.0.9 and am having an issue using POLCA to polish a Flye assembly with Illumina reads where it prints the following error:
From what I can tell, the .vcf file is not being generated and this causes the fix_consensus_from_vcf.pl script to crash? I've run this command in this workflow many times and have not had any issues except for this one assembly file. There's 6 contigs in this assembly file and I can run POLCA on each contig individually without issue. I'm not sure how to diagnose the problem from this error message; it's not clear to me if there is a problem with my assembly file or a bug in the program and would appreciate any help.
May be related to #308
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