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[BUG] #146

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fork16 opened this issue Sep 14, 2023 · 0 comments
Open

[BUG] #146

fork16 opened this issue Sep 14, 2023 · 0 comments

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@fork16
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fork16 commented Sep 14, 2023

  • ribotricer version: 1.3.3
  • Python version: 3.10.12
  • Operating System: GNU bash, version 5.0.17(1)-release (x86_64-pc-linux-gnu)

Description

I have two sample to detect orfs. One is done but another gets following bug.

What I Did


ribotricer detect-orfs --bam ${file} --ribotricer_index ${ribotricer_index_tsv} --prefix ${file/bam/} --report_all --phase_score_cutoff 0
Sep 14 10:25:03 ..... started ribotricer detect-orfs
Sep 14 10:25:03 ... started parsing ribotricer index file
Sep 14 10:25:07 ... started inferring experimental design                                                                               
Sep 14 10:25:07 ... started reading bam file
Sep 14 10:25:10 ... started plotting read length distribution                                                                           
Sep 14 10:25:10 ... started calculating metagene profiles. This may take a long time...
                                    
Sep 14 10:25:10 ... started plotting metagene profiles
Sep 14 10:25:10 ... started inferring P-site offsets
WARNING: no periodic read length found... using cutoff 0.0

The difference between the two files is not significant, but read counts of successful one is more than another one.
image
image

Is this an error caused by the read counts?

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