Ribotricer Output

[bshim181]

Hello,

I am trying to merge two list of ORFs predicted by the RibORF algorithm and Ribotricer Algorithm. I am aware that RibORF predicts ORFs at the transcript level while the Ribotricer predicts ORFs at the exonal level. I would like to create a bed file where it encompasses predictions from both outputs. Is there way to merge two ORFs predicted at different level (transcript vs exonal)?

Is there a feature where I could transform the exonal predictions by RibORF to transcript level predictions? Also does it make sense to input an offset corrected bam file (corrected by ribORF) for ribotricer input?

[saketkc]

My suggestion would be to use the same annotation file for both. That is, when you generate ribotricer index, you should be able to modify it and use as in index to RibORF. I haven't used RibORF for a while, but when we benchmarked, this is the strategy we used.

[bshim181]

This is the ORF annotation file for ribotricer. I was wondering what the coordinates of the last column stand for.

This is the ORF annotation for the same transcript_ID by ribORF. I was wondering how we would know the start and end coordinate of the parent transcript (not each of its isoforms) which is specified in the ribORF annotations.

[saketkc]

The last column are the coordinates of the that ORF - comma separated values indicate the start and end points (these are exons and hence not continuous).

Hope that helps!

[bshim181]

Hello,

I have been working to transform the Ribotricer index to the RibORF index. I have noticed that the exon boundaries coordinates are not multiple of 3 while RibORF index demonstrates transcript level annotations with transcript length being multiple of 3. Would this cause issues? I have generated the prediction with RIbORF algorithm with the transformed index. It is, however, difficult to verify whether it was converted accurately.

I was wondering if you have converted RibORF annotations or the code use to generate the conversion from one index to the another, would you be able to share that with us? or would that be difficult?