Skip to content
New issue

Have a question about this project? Sign up for a free GitHub account to open an issue and contact its maintainers and the community.

By clicking “Sign up for GitHub”, you agree to our terms of service and privacy statement. We’ll occasionally send you account related emails.

Already on GitHub? Sign in to your account

Rbk mode #197

Open
wants to merge 3 commits into
base: master
Choose a base branch
from
Open

Rbk mode #197

wants to merge 3 commits into from

Conversation

GeorgetteTanner
Copy link

  • Added a new mode "rbk" (rapid barcode kit) to replicate the read distribution seen in Nanopore amplicon rbk data, where forward reads are enriched at the amplicon 3' end and reverse reads are enriched at the 5' end.
  • Stopped simulating unaligned flanking head and tail regions in all modes, with option to turn it back on if required.
  • Fixed an issue where when a minimum read length parameter was set in 'genome' or 'rbk' mode, if a generated read was too short it was repeatedly reset and mutated until it reached the length threshold. This resulted in an unrealistically high number of reads just longer than the minimum threshold. Now the read is just discarded and a new read length randomly sampled.
  • Added a new pre-trained error model: amplicon-rbk

Sign up for free to join this conversation on GitHub. Already have an account? Sign in to comment
Labels
None yet
Projects
None yet
Development

Successfully merging this pull request may close these issues.

None yet

1 participant