Dynast: Inclusive and efficient quantification of labeling and splicing RNAs for time-resolved metabolic labeling based scRNA-seq experiments

Getting started - Pipeline usage - Technical information - Full documentation

Dynast is a command-line pipeline that preprocesses data from metabolic labeling scRNA-seq experiments and quantifies the following four mRNA species: unlabeled unspliced, unlabeled spliced, labeled unspliced and labeled spliced. In addition, dynast can perform statistical estimation of these species through expectation maximization (EM) and Bayesian inference.

Key features

• Novel consensus-calling algorithm to identify the most probable base at each sequenced position.
• Complete quantification of labeling and splicing of RNA.
  • Unlabeled unspliced, unlabeled spliced, labeled unspliced, labeled spliced species quantification.
  • Highly paralellized and optimized pipeline procedures.
• Statistical estimation procedure that corrects the observed unlabeled and labeled counts via a Bayesian inference framework.