Hybrid assembly using low quality HiFi reads.

[javibio-git]

Hi,

I am helping some collaborators who recently got some HiFi reads to do a de novo genome assembly. Unfortunately, the quality of their DNA was below the standards and they end up having a coverage of ~5X with a mean read length of ~5kb.

The question is if this data is still usable? I am thinking that conducting a hybrid assembly using high coverage short reads we could still get a decent genome assembly. Would you consider this worth trying?

Thank you!

I attach an example of the read length distribution: