Skip to content
New issue

Have a question about this project? Sign up for a free GitHub account to open an issue and contact its maintainers and the community.

By clicking “Sign up for GitHub”, you agree to our terms of service and privacy statement. We’ll occasionally send you account related emails.

Already on GitHub? Sign in to your account

Assembly contiguity & sequencing depth #345

Open
TymekPieszko opened this issue Dec 11, 2022 · 0 comments
Open

Assembly contiguity & sequencing depth #345

TymekPieszko opened this issue Dec 11, 2022 · 0 comments

Comments

@TymekPieszko
Copy link

Hello,

I have two genomic libraries with mean read depth of 24x and 120x. Surprisingly, after megahit assembly, the lower-depth assembly is more contiguous (twice as few contigs, twice as high N50). Are there any possible explanations for that in terms of the assembly algorithm? Subsampling reads from the high-depth library again gives improved contiguity - what's going on?

All the best, thanks for any help in advance!

Sign up for free to join this conversation on GitHub. Already have an account? Sign in to comment
Labels
None yet
Projects
None yet
Development

No branches or pull requests

1 participant