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I'm having difficulty clustering using profiles when following the instructions in the wiki. Specifically I'm referring to this section:
# extract consensus sequences from profiles
mmseqs profile2consensus profileDB1 profileDB1_consensus
# search with profiles against consensus sequences of seqDB1
mmseqs search profileDB1 profileDB1_consensus resultDB2 tmp --add-self-matches -a # Add your cluster criteria here
# cluster the results
mmseqs clust profileDB1 resultDB2 profileDB1_clu
I can run mmseqs search without issue but when I run mmseqs clust I get the following error:
Create directory /final/db_cluster/low_1/Genus02938/Genus02938_DB
cluster /final/db_profile/low_1/Genus02938/Genus02938_DB /final/db_profile_vs_consensus/low_1/Genus02938/Genus02938_DB /final/db_cluster/low_1/Genus02938/Genus02938_DB
MMseqs Version: 15.6f452
Substitution matrix aa:blosum62.out,nucl:nucleotide.out
Seed substitution matrix aa:VTML80.out,nucl:nucleotide.out
Sensitivity 4
k-mer length 0
Target search mode 0
k-score seq:2147483647,prof:2147483647
Alphabet size aa:21,nucl:5
Max sequence length 65535
Max results per query 20
Split database 0
Split mode 2
Split memory limit 0
Coverage threshold 0.8
Coverage mode 0
Compositional bias 1
Compositional bias 1
Diagonal scoring true
Exact k-mer matching 0
Mask residues 1
Mask residues probability 0.9
Mask lower case residues 0
Minimum diagonal score 15
Selected taxa
Include identical seq. id. false
Spaced k-mers 1
Preload mode 0
Pseudo count a substitution:1.100,context:1.400
Pseudo count b substitution:4.100,context:5.800
Spaced k-mer pattern
Local temporary path
Threads 144
Compressed 0
Verbosity 3
Add backtrace false
Alignment mode 3
Alignment mode 0
Allow wrapped scoring false
E-value threshold 0.001
Seq. id. threshold 0
Min alignment length 0
Seq. id. mode 0
Alternative alignments 0
Max reject 2147483647
Max accept 2147483647
Score bias 0
Realign hits false
Realign score bias -0.2
Realign max seqs 2147483647
Correlation score weight 0
Gap open cost aa:11,nucl:5
Gap extension cost aa:1,nucl:2
Zdrop 40
Rescore mode 0
Remove hits by seq. id. and coverage false
Sort results 0
Cluster mode 0
Max connected component depth 1000
Similarity type 2
Weight file name
Cluster Weight threshold 0.9
Single step clustering false
Cascaded clustering steps 3
Cluster reassign false
Remove temporary files false
Force restart with latest tmp false
MPI runner
k-mers per sequence 21
Scale k-mers per sequence aa:0.000,nucl:0.200
Adjust k-mer length false
Shift hash 67
Include only extendable false
Skip repeating k-mers false
Set cluster sensitivity to -s 6.000000
Set cluster mode SET COVER
Set cluster iterations to 3
/final/db_profile_vs_consensus/low_1/Genus02938/Genus02938_DB.dbtype exists already!
Yes, /final/db_profile_vs_consensus/low_1/Genus02938/Genus02938_DB.dbtype already exists; it was created by mmseqs search. I'm not sure why mmseqs clust cares? Do you have any ideas - I can't figure this out. Many thanks!!
The text was updated successfully, but these errors were encountered:
schmittel
changed the title
'could not copy file' error when clustering using profiles
.dbtype already exists error when clustering using profiles
May 6, 2024
Hi,
I'm having difficulty clustering using profiles when following the instructions in the wiki. Specifically I'm referring to this section:
I can run
mmseqs search
without issue but when I runmmseqs clust
I get the following error:Yes,
/final/db_profile_vs_consensus/low_1/Genus02938/Genus02938_DB.dbtype
already exists; it was created bymmseqs search
. I'm not sure whymmseqs clust
cares? Do you have any ideas - I can't figure this out. Many thanks!!The text was updated successfully, but these errors were encountered: