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KEGG annotations - the number is much lower number than expected #285

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tvtv195 opened this issue Apr 25, 2024 · 1 comment
Open

KEGG annotations - the number is much lower number than expected #285

tvtv195 opened this issue Apr 25, 2024 · 1 comment
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@tvtv195
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tvtv195 commented Apr 25, 2024

Dear bakta team,
We ran bakta on several bacterial genomes, with >50% estimated compl. and <10% contam., to obtain KEGG annotations.
Bakta ran just fine, without any issues, e.g.:

bakta --db /gpfs/gpfs1/scratch/cb761220/databases/bakta_db_2024/db
-o /scratch/cb761203/02.analysis/13.module13/01.bakta/ -v
/scratch/cb761203/02.analysis/12.module12/bins_hq/sample.bin.35.fa

However, we only got between 0 and 17 KEGG annotations (K0 ID) per genome.
For example, a very small bacterial genome of 531,276 bp had 517 CDS but not even a single KEGG annotation.

Is there something wrong with the way bakta assigns KEGG annotations?
Best,
Chris

@tvtv195 tvtv195 added the bug Something isn't working label Apr 25, 2024
@tvtv195 tvtv195 changed the title KEGG annotations - much lower number than expected KEGG annotations - the number is much lower number than expected Apr 26, 2024
@oschwengers
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Hi Chris, and thanks for reaching out. Based on the command line above, I guess you're working on a MAG. Depending on the species it could simply be the case that there are only few to no genes similar to those stored in KEGG. Could you provide some information about how many UniRef90-annotated genes and how many hypotheticals you get ?

@oschwengers oschwengers added question Further information is requested and removed bug Something isn't working labels Apr 28, 2024
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