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NEWS.md

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porefile 1.0.0.9000 (xx/03/2023)

  • Refactoring. Rscripts are now in bin/ folder.
  • Added seqkit to environment.yml
  • Added reduceSilva process to remove eukaryota and phage sequences. Deactivate it with --fullSilva flag. This reduction step improves 16S classification and possibly reduces the running time a bit.
  • Removed seqtk in favour of seqkit for fasta/fastq manipulation.
  • Exposed the --headcrop and --tailcrop parameters of NanoFilt.
  • Synonyms mapping file now gets published in results folder.
  • Parameters are displayed at the begining of the run.
  • Nanofilt quality default is now 10 (was 8 before).
  • Added the Polishing workflow. Related: added megan_topPercentPolish params.
  • Now read assignments get published.
  • Removed Last, LastTrain, and Megablast workflows since added complexity to the code and any of them wasn't good enough as Minimap2 workflow.
  • New --help.
  • New README.md.
  • New container, now based on micromamba instead of conda.
  • New process GetVersions to print a file with the versions of the dependencies used (installed in the container).
  • Added --removeChimeras to activate chimera-removing step with (Yacrd).
  • Added PacBio management if user provides --minimap2_x map-pb or --minimap2_x map-hifi

porefile 0.2.2 14/12/2022

  • Minor fix in "nagual" config, which was using a "dev" tagged docker container. Now it uses the "latest".

porefile 0.2.1 24/11/2022

  • Fixed wrong parameter documentation. "--megan_topPercent" was documented as "--megan_lcaTopPercent", so this parameter was completely ignored and the default was always used instead.
  • Added parameters sanity checking. This is to avoid ignoring misspelled parameters.

porefile 0.2.0 15/11/2022

  • Merge s138: Now Porefiles only uses SILVA files, not needed MEGAN mapping files any more. Uses latest SILVA version (138.1).
  • Fix #12: If AutoMap returns empty, this barcode is discarded.
  • Added "--porechop_extra_end_trim" parameter to allow trimming n bases from the end of the amplicon in case adapters were added.
  • Minor improvements in error handling.
  • Updated documentation and scheme figure.
  • Updated --help

porefile 0.1.1 29/03/2022

  • General changes
  • Updated environment.yml
  • Updated Dockerfile
  • Updated container to verion 0.1.1

porefile 0.1.0 29/10/2020

  • Initial release