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I have a long read (ONT) that I am aligning to the reference genome using Minimap2 -x splice mode.
Minimap2 is generating a 1bp exon as part of the alignment of this alignment:
Note the 1bp match (111186N, 1=, 182183N). Why is there such a single basepair match? It looks like Minimap2 is trying to force splicing on this read since the total of the two surrounding splice gaps is >200Kbp
The text was updated successfully, but these errors were encountered:
I have a long read (ONT) that I am aligning to the reference genome using Minimap2
-x splice
mode.Minimap2 is generating a 1bp exon as part of the alignment of this alignment:
This generates the following PAF alignment:
Note the 1bp match (
111186N
,1=
,182183N
). Why is there such a single basepair match? It looks like Minimap2 is trying to force splicing on this read since the total of the two surrounding splice gaps is >200KbpThe text was updated successfully, but these errors were encountered: