How to use data obtained from other software to start from the middle of the steps of SqueezeMeta and run the subsequent steps?

[mojiefei]

I used funannotate software to predict the genes in the assembled metagenomic sequences and obtained the corresponding result files. I want to run steps 4 to 16 in SqueezeMeta, using these result files as input files directly.

However, I only saw in the software instructions that SqueezeMeta supports restarting from a certain step or re-running a certain script. These seem to require using the intermediate output files from the initial run of SqueezeMeta as input to the steps needing re-run. And the input of SqueezeMeta is only the paired-end sequence files (.fq.gz) obtained by sequencing.

So, what can I do to use the result files of funannotate software as input of steps 4 to 16 in SqueezeMeta?

[fpusan]

Hi!
I guess you could write a parser that parses the results of the funannotate software to a format similar to the intermediate files used by SqueezeMeta (which is documented in the PDF manual).
You can also create an empty SqueezeMeta run by adding the flag --empty, this would allow you to have the project's "skeleton", so to speak so you could then restart it from whatever step you want (as long as the required intermediate files are in place).
Hope this is helpful, I can not provide much more help as I am not familiar with the funannotate output.

[jtamames]

Hello
If the only information you want to use is a new gene prediction, you could replace the 03.faa (aa sequences), 03.fna (nucleotide sequences) and 03.gff (information for ORFs) file for new ones you have from funannotate (keeping the ORF naming schema of SqueezeMeta, which is contigname_ORFinitpos_ORFendpos).
The restart in step 4. You would also need to run the rest of steps, not only 16, for getting abundance measures for ORFs, new annotations, etc.
Hope it helps.
Best,
J

[mojiefei]

Hello If the only information you want to use is a new gene prediction, you could replace the 03.faa (aa sequences), 03.fna (nucleotide sequences) and 03.gff (information for ORFs) file for new ones you have from funannotate (keeping the ORF naming schema of SqueezeMeta, which is contigname_ORFinitpos_ORFendpos). The restart in step 4. You would also need to run the rest of steps, not only 16, for getting abundance measures for ORFs, new annotations, etc. Hope it helps. Best, J

@jtamames

I did what you said, but SqueezeMeta reported an error in Step 9. The error displayed by syslog was "Stopping in STEP9 -> 09.summarycontigs3.pl. File /hdd/mojf/output/B11_Eukaryote_TEST/intermediate/09.B11_Eukaryote_TEST.contiglog is empty!"

The 01.B11_Eukaryote_TEST.fasta was eukaryotic contigs isolated using EukRep from the files obtained through Step 1 of SqueezeMeta. The first few lines of 01.B11_Eukaryote_TEST.fasta were as follows.

The first 10 lines of 03.B11_Eukaryote_TEST.faa from funannotate were as follows.

The first 10 lines of 03.B11_Eukaryote_TEST.fna from funannotate were as follows.

The first 10 lines of 03.B11_Eukaryote_TEST.gff from funannotate were as follows.

I moved these four files into results, and restart SqueezeMeta using 02.rnas.pl B11_Eukaryote_TEST, and then SqueezeMeta.pl --restart -p B11_Eukaryote_TEST -step 4 --force_overwrite. The complete syslog is as follows.

Running barrnap for Bacteria: /home/gfz3/miniconda3/envs/SqueezeMeta/SqueezeMeta/bin/barrnap --quiet --threads 32 --kingdom bac --reject 0.1 /hdd/mojf/output/B11_Eukaryote_TEST/intermediate/02.B11_Eukaryote_TEST.maskedrna.fasta --dbdir /database/SqueezeMetaDB/db > /hdd/mojf/output/B11_Eukaryote_TEST/temp/bac.gff
Running barrnap for Archaea: /home/gfz3/miniconda3/envs/SqueezeMeta/SqueezeMeta/bin/barrnap --quiet --threads 32 --kingdom arc --reject 0.1 /hdd/mojf/output/B11_Eukaryote_TEST/intermediate/02.B11_Eukaryote_TEST.maskedrna.fasta --dbdir /database/SqueezeMetaDB/db > /hdd/mojf/output/B11_Eukaryote_TEST/temp/arc.gff
Running barrnap for Eukaryote: /home/gfz3/miniconda3/envs/SqueezeMeta/SqueezeMeta/bin/barrnap --quiet --threads 32 --kingdom euk --reject 0.1 /hdd/mojf/output/B11_Eukaryote_TEST/intermediate/02.B11_Eukaryote_TEST.maskedrna.fasta --dbdir /database/SqueezeMetaDB/db > /hdd/mojf/output/B11_Eukaryote_TEST/temp/euk.gff
Running barrnap for Mitochondrial: /home/gfz3/miniconda3/envs/SqueezeMeta/SqueezeMeta/bin/barrnap --quiet --threads 32 --kingdom mito --reject 0.1 /hdd/mojf/output/B11_Eukaryote_TEST/intermediate/02.B11_Eukaryote_TEST.maskedrna.fasta --dbdir /database/SqueezeMetaDB/db > /hdd/mojf/output/B11_Eukaryote_TEST/temp/mito.gff
Running RDP classifier: java -jar /home/gfz3/miniconda3/envs/SqueezeMeta/SqueezeMeta/lib/classifier/classifier.jar classify /hdd/mojf/output/B11_Eukaryote_TEST/temp/16S.fasta -o /hdd/mojf/output/B11_Eukaryote_TEST/temp/16S.out -f filterbyconf
Running Aragorn: /home/gfz3/miniconda3/envs/SqueezeMeta/SqueezeMeta/bin/aragorn -w /hdd/mojf/output/B11_Eukaryote_TEST/intermediate/02.B11_Eukaryote_TEST.maskedrna.fasta -o /hdd/mojf/output/B11_Eukaryote_TEST/temp/trnas.aragorn
Creating new gff file: cat /hdd/mojf/output/B11_Eukaryote_TEST/temp/*gff.mod > /hdd/mojf/output/B11_Eukaryote_TEST/temp/02.B11_Eukaryote_TEST.rna.gff
Diamond block size set to 16 (Free Mem 705.85 Gb)
  Working with taxonomy database in /database/SqueezeMetaDB/db/nr.dmnd
Running Diamond for taxa: /home/gfz3/miniconda3/envs/SqueezeMeta/SqueezeMeta/bin/diamond blastp -q /hdd/mojf/output/B11_Eukaryote_TEST/results/03.B11_Eukaryote_TEST.faa -p 32 -d /database/SqueezeMetaDB/db/nr.dmnd -e 0.001 --id 40 -f tab -b 16 -o /hdd/mojf/output/B11_Eukaryote_TEST/intermediate/04.B11_Eukaryote_TEST.nr.diamond > /hdd/mojf/output/B11_Eukaryote_TEST/temp/diamond.nr.log 2>&1
Diamond block size set to 16 (Free Mem 671.82 Gb)
  Working with taxonomy database in /database/SqueezeMetaDB/db/nr.dmnd
Running Diamond for taxa: /home/gfz3/miniconda3/envs/SqueezeMeta/SqueezeMeta/bin/diamond blastp -q /hdd/mojf/output/B11_Eukaryote_TEST/results/03.B11_Eukaryote_TEST.faa -p 32 -d /database/SqueezeMetaDB/db/nr.dmnd -e 0.001 --id 40 -f tab -b 16 -o /hdd/mojf/output/B11_Eukaryote_TEST/intermediate/04.B11_Eukaryote_TEST.nr.diamond > /hdd/mojf/output/B11_Eukaryote_TEST/temp/diamond.nr.log 2>&1
Running Diamond for COGs: /home/gfz3/miniconda3/envs/SqueezeMeta/SqueezeMeta/bin/diamond blastp -q /hdd/mojf/output/B11_Eukaryote_TEST/results/03.B11_Eukaryote_TEST.faa -p 32 -d /database/SqueezeMetaDB/db/eggnog -e 0.001 --id 30 --quiet -b 16 -f 6 qseqid qlen sseqid slen pident length evalue bitscore qstart qend sstart send -o /hdd/mojf/output/B11_Eukaryote_TEST/intermediate/04.B11_Eukaryote_TEST.eggnog.diamond
Running Diamond for KEGG: /home/gfz3/miniconda3/envs/SqueezeMeta/SqueezeMeta/bin/diamond blastp -q /hdd/mojf/output/B11_Eukaryote_TEST/results/03.B11_Eukaryote_TEST.faa -p 32 -d /database/SqueezeMetaDB/db/keggdb -e 0.001 --id 30 --quiet -b 16 -f 6 qseqid qlen sseqid slen pident length evalue bitscore qstart qend sstart send -o /hdd/mojf/output/B11_Eukaryote_TEST/intermediate/04.B11_Eukaryote_TEST.kegg.diamond

[55 minutes, 9 seconds]: STEP5 -> 05.run_hmmer.pl
Running HMMER3 for Pfam: /home/gfz3/miniconda3/envs/SqueezeMeta/SqueezeMeta/bin/hmmer/hmmsearch --domtblout /hdd/mojf/output/B11_Eukaryote_TEST/intermediate/05.B11_Eukaryote_TEST.pfam.hmm -E 1e-10 --cpu 32 /database/SqueezeMetaDB/db/Pfam-A.hmm /hdd/mojf/output/B11_Eukaryote_TEST/results/03.B11_Eukaryote_TEST.faa > /dev/null 2>&1

[1 hours, 6 minutes, 43 seconds]: STEP6 -> 06.lca.pl
  Splitting Diamond file
  Total lines in Diamond: 141918; Allocating 4434 in 32 threads
  Opening file 1 in line  (estimated in 4434)
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  Starting multithread LCA in 32 threads
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  Creating /hdd/mojf/output/B11_Eukaryote_TEST/results/06.B11_Eukaryote_TEST.fun3.tax.wranks file: cat /hdd/mojf/output/B11_Eukaryote_TEST/temp/fun3tax_1.wranks /hdd/mojf/output/B11_Eukaryote_TEST/temp/fun3tax_2.wranks /hdd/mojf/output/B11_Eukaryote_TEST/temp/fun3tax_3.wranks /hdd/mojf/output/B11_Eukaryote_TEST/temp/fun3tax_4.wranks /hdd/mojf/output/B11_Eukaryote_TEST/temp/fun3tax_5.wranks /hdd/mojf/output/B11_Eukaryote_TEST/temp/fun3tax_6.wranks /hdd/mojf/output/B11_Eukaryote_TEST/temp/fun3tax_7.wranks /hdd/mojf/output/B11_Eukaryote_TEST/temp/fun3tax_8.wranks /hdd/mojf/output/B11_Eukaryote_TEST/temp/fun3tax_9.wranks /hdd/mojf/output/B11_Eukaryote_TEST/temp/fun3tax_10.wranks /hdd/mojf/output/B11_Eukaryote_TEST/temp/fun3tax_11.wranks /hdd/mojf/output/B11_Eukaryote_TEST/temp/fun3tax_12.wranks /hdd/mojf/output/B11_Eukaryote_TEST/temp/fun3tax_13.wranks /hdd/mojf/output/B11_Eukaryote_TEST/temp/fun3tax_14.wranks /hdd/mojf/output/B11_Eukaryote_TEST/temp/fun3tax_15.wranks /hdd/mojf/output/B11_Eukaryote_TEST/temp/fun3tax_16.wranks /hdd/mojf/output/B11_Eukaryote_TEST/temp/fun3tax_17.wranks /hdd/mojf/output/B11_Eukaryote_TEST/temp/fun3tax_18.wranks /hdd/mojf/output/B11_Eukaryote_TEST/temp/fun3tax_19.wranks /hdd/mojf/output/B11_Eukaryote_TEST/temp/fun3tax_20.wranks /hdd/mojf/output/B11_Eukaryote_TEST/temp/fun3tax_21.wranks /hdd/mojf/output/B11_Eukaryote_TEST/temp/fun3tax_22.wranks /hdd/mojf/output/B11_Eukaryote_TEST/temp/fun3tax_23.wranks /hdd/mojf/output/B11_Eukaryote_TEST/temp/fun3tax_24.wranks /hdd/mojf/output/B11_Eukaryote_TEST/temp/fun3tax_25.wranks /hdd/mojf/output/B11_Eukaryote_TEST/temp/fun3tax_26.wranks /hdd/mojf/output/B11_Eukaryote_TEST/temp/fun3tax_27.wranks /hdd/mojf/output/B11_Eukaryote_TEST/temp/fun3tax_28.wranks /hdd/mojf/output/B11_Eukaryote_TEST/temp/fun3tax_29.wranks /hdd/mojf/output/B11_Eukaryote_TEST/temp/fun3tax_30.wranks /hdd/mojf/output/B11_Eukaryote_TEST/temp/fun3tax_31.wranks /hdd/mojf/output/B11_Eukaryote_TEST/temp/fun3tax_32.wranks > /hdd/mojf/output/B11_Eukaryote_TEST/results/06.B11_Eukaryote_TEST.fun3.tax.wranks
  Creating /hdd/mojf/output/B11_Eukaryote_TEST/results/06.B11_Eukaryote_TEST.fun3.tax.noidfilter.wranks file: cat /hdd/mojf/output/B11_Eukaryote_TEST/temp/fun3tax_1.noidfilter.wranks /hdd/mojf/output/B11_Eukaryote_TEST/temp/fun3tax_2.noidfilter.wranks /hdd/mojf/output/B11_Eukaryote_TEST/temp/fun3tax_3.noidfilter.wranks /hdd/mojf/output/B11_Eukaryote_TEST/temp/fun3tax_4.noidfilter.wranks /hdd/mojf/output/B11_Eukaryote_TEST/temp/fun3tax_5.noidfilter.wranks /hdd/mojf/output/B11_Eukaryote_TEST/temp/fun3tax_6.noidfilter.wranks /hdd/mojf/output/B11_Eukaryote_TEST/temp/fun3tax_7.noidfilter.wranks /hdd/mojf/output/B11_Eukaryote_TEST/temp/fun3tax_8.noidfilter.wranks /hdd/mojf/output/B11_Eukaryote_TEST/temp/fun3tax_9.noidfilter.wranks /hdd/mojf/output/B11_Eukaryote_TEST/temp/fun3tax_10.noidfilter.wranks /hdd/mojf/output/B11_Eukaryote_TEST/temp/fun3tax_11.noidfilter.wranks /hdd/mojf/output/B11_Eukaryote_TEST/temp/fun3tax_12.noidfilter.wranks /hdd/mojf/output/B11_Eukaryote_TEST/temp/fun3tax_13.noidfilter.wranks /hdd/mojf/output/B11_Eukaryote_TEST/temp/fun3tax_14.noidfilter.wranks /hdd/mojf/output/B11_Eukaryote_TEST/temp/fun3tax_15.noidfilter.wranks /hdd/mojf/output/B11_Eukaryote_TEST/temp/fun3tax_16.noidfilter.wranks /hdd/mojf/output/B11_Eukaryote_TEST/temp/fun3tax_17.noidfilter.wranks /hdd/mojf/output/B11_Eukaryote_TEST/temp/fun3tax_18.noidfilter.wranks /hdd/mojf/output/B11_Eukaryote_TEST/temp/fun3tax_19.noidfilter.wranks /hdd/mojf/output/B11_Eukaryote_TEST/temp/fun3tax_20.noidfilter.wranks /hdd/mojf/output/B11_Eukaryote_TEST/temp/fun3tax_21.noidfilter.wranks /hdd/mojf/output/B11_Eukaryote_TEST/temp/fun3tax_22.noidfilter.wranks /hdd/mojf/output/B11_Eukaryote_TEST/temp/fun3tax_23.noidfilter.wranks /hdd/mojf/output/B11_Eukaryote_TEST/temp/fun3tax_24.noidfilter.wranks /hdd/mojf/output/B11_Eukaryote_TEST/temp/fun3tax_25.noidfilter.wranks /hdd/mojf/output/B11_Eukaryote_TEST/temp/fun3tax_26.noidfilter.wranks /hdd/mojf/output/B11_Eukaryote_TEST/temp/fun3tax_27.noidfilter.wranks /hdd/mojf/output/B11_Eukaryote_TEST/temp/fun3tax_28.noidfilter.wranks /hdd/mojf/output/B11_Eukaryote_TEST/temp/fun3tax_29.noidfilter.wranks /hdd/mojf/output/B11_Eukaryote_TEST/temp/fun3tax_30.noidfilter.wranks /hdd/mojf/output/B11_Eukaryote_TEST/temp/fun3tax_31.noidfilter.wranks /hdd/mojf/output/B11_Eukaryote_TEST/temp/fun3tax_32.noidfilter.wranks  > /hdd/mojf/output/B11_Eukaryote_TEST/results/06.B11_Eukaryote_TEST.fun3.tax.noidfilter.wranks
  Removing temporaty diamond files in /hdd/mojf/output/B11_Eukaryote_TEST/temp

[1 hours, 9 minutes, 2 seconds]: STEP7 -> 07.fun3assign.pl
  Reading COGs hits from /hdd/mojf/output/B11_Eukaryote_TEST/intermediate/04.B11_Eukaryote_TEST.eggnog.diamond
  Output in /hdd/mojf/output/B11_Eukaryote_TEST/results/07.B11_Eukaryote_TEST.fun3.cog
  Reading KEGG hits from /hdd/mojf/output/B11_Eukaryote_TEST/intermediate/04.B11_Eukaryote_TEST.kegg.diamond
  Output in /hdd/mojf/output/B11_Eukaryote_TEST/results/07.B11_Eukaryote_TEST.fun3.kegg
  Reading pfam hits from /hdd/mojf/output/B11_Eukaryote_TEST/intermediate/05.B11_Eukaryote_TEST.pfam.hmm
  Output in /hdd/mojf/output/B11_Eukaryote_TEST/results/07.B11_Eukaryote_TEST.fun3.pfam

[1 hours, 9 minutes, 2 seconds]: STEP9 -> 09.summarycontigs3.pl
  Reading taxa for genes from /hdd/mojf/output/B11_Eukaryote_TEST/results/06.B11_Eukaryote_TEST.fun3.tax.wranks
  Reading results without eukaryotic filter from /hdd/mojf/output/B11_Eukaryote_TEST/results/06.B11_Eukaryote_TEST.fun3.tax.noidfilter.wranks
  Writing output to /hdd/mojf/output/B11_Eukaryote_TEST/temp/09.B11_Eukaryote_TEST.allorfs and /hdd/mojf/output/B11_Eukaryote_TEST/intermediate/09.B11_Eukaryote_TEST.contiglog
  Reading taxa for genes from /hdd/mojf/output/B11_Eukaryote_TEST/results/06.B11_Eukaryote_TEST.fun3.tax.noidfilter.wranks
  Reading results without eukaryotic filter from /hdd/mojf/output/B11_Eukaryote_TEST/results/06.B11_Eukaryote_TEST.fun3.tax.noidfilter.wranks
  Writing output to /hdd/mojf/output/B11_Eukaryote_TEST/temp/09.B11_Eukaryote_TEST.allorfs.noidfilter and /hdd/mojf/output/B11_Eukaryote_TEST/intermediate/09.B11_Eukaryote_TEST.contiglog.noidfilter
Stopping in STEP9 -> 09.summarycontigs3.pl. File /hdd/mojf/output/B11_Eukaryote_TEST/intermediate/09.B11_Eukaryote_TEST.contiglog is empty!
_____________

System information:
Linux dell 6.5.0-26-generic #26~22.04.1-Ubuntu SMP PREEMPT_DYNAMIC Tue Mar 12 10:22:43 UTC 2 x86_64 x86_64 x86_64 GNU/Linux
_____________

Tree for the project:
[4.0K Apr  9 19:48]  /hdd/mojf/output/B11_Eukaryote_TEST
├── [  35 Apr  9 19:48]  creator.txt
├── [4.0K Apr  9 19:48]  data
│   ├── [  56 Apr  9 19:48]  00.B11_Eukaryote_TEST.samples
│   └── [4.0K Apr  9 19:48]  raw_fastq
│       ├── [3.4G Apr  9 19:48]  par1.fastq.gz
│       └── [2.2G Apr  9 19:49]  par2.fastq.gz
├── [4.0K Apr  9 19:48]  ext_tables
├── [4.0K Apr  9 22:15]  intermediate
│   ├── [ 12M Apr  9 21:02]  02.B11_Eukaryote_TEST.maskedrna.fasta
│   ├── [9.7M Apr  9 22:00]  04.B11_Eukaryote_TEST.eggnog.diamond
│   ├── [7.2M Apr  9 22:00]  04.B11_Eukaryote_TEST.kegg.diamond
│   ├── [9.1M Apr  9 21:59]  04.B11_Eukaryote_TEST.nr.diamond
│   ├── [2.4M Apr  9 22:12]  05.B11_Eukaryote_TEST.pfam.hmm
│   ├── [ 289 Apr  9 22:15]  09.B11_Eukaryote_TEST.contiglog
│   ├── [ 297 Apr  9 22:15]  09.B11_Eukaryote_TEST.contiglog_allranks
│   ├── [ 308 Apr  9 22:15]  09.B11_Eukaryote_TEST.contiglog_allranks.noidfilter
│   ├── [ 300 Apr  9 22:15]  09.B11_Eukaryote_TEST.contiglog.noidfilter
│   ├── [4.0K Apr  9 19:48]  binners
│   └── [  56 Apr  9 21:05]  DB_BUILD_DATE
├── [ 904 Apr  9 22:12]  methods.txt
├── [3.1K Apr  9 19:48]  parameters.pl
├── [  36 Apr  9 19:48]  progress
├── [4.0K Apr  9 22:14]  results
│   ├── [ 12M Apr  9 20:59]  01.B11_Eukaryote_TEST.fasta
│   ├── [ 130 Apr  9 21:02]  02.B11_Eukaryote_TEST.16S.txt
│   ├── [ 171 Apr  9 21:01]  02.B11_Eukaryote_TEST.rnas
│   ├── [2.7K Apr  9 21:02]  02.B11_Eukaryote_TEST.trnas
│   ├── [ 28K Apr  9 21:02]  02.B11_Eukaryote_TEST.trnas.fasta
│   ├── [3.0M Apr  9 20:43]  03.B11_Eukaryote_TEST.faa
│   ├── [8.8M Apr  9 20:57]  03.B11_Eukaryote_TEST.fna
│   ├── [611K Apr  9 20:34]  03.B11_Eukaryote_TEST.gff
│   ├── [952K Apr  9 22:14]  06.B11_Eukaryote_TEST.fun3.tax.noidfilter.wranks
│   ├── [932K Apr  9 22:14]  06.B11_Eukaryote_TEST.fun3.tax.wranks
│   ├── [181K Apr  9 22:14]  07.B11_Eukaryote_TEST.fun3.cog
│   ├── [118K Apr  9 22:14]  07.B11_Eukaryote_TEST.fun3.kegg
│   └── [348K Apr  9 22:14]  07.B11_Eukaryote_TEST.fun3.pfam
├── [8.2K Apr  9 19:48]  SqueezeMeta_conf.pl
├── [ 14K Apr  9 22:15]  syslog
└── [4.0K Apr  9 22:15]  temp
    ├── [7.1K Apr  9 21:02]  02.B11_Eukaryote_TEST.rna.gff
    ├── [ 290 Apr  9 22:15]  09.B11_Eukaryote_TEST.allorfs
    ├── [ 301 Apr  9 22:15]  09.B11_Eukaryote_TEST.allorfs.noidfilter
    ├── [   0 Apr  9 21:01]  16S.fasta
    ├── [  40 Apr  9 21:02]  16S.out
    ├── [  16 Apr  9 21:01]  arc.gff
    ├── [  16 Apr  9 21:01]  arc.gff.mod
    ├── [ 109 Apr  9 21:01]  bac.gff
    ├── [ 134 Apr  9 21:01]  bac.gff.mod
    ├── [ 13K Apr  9 21:59]  diamond.nr.log
    ├── [  16 Apr  9 21:02]  euk.gff
    ├── [  16 Apr  9 21:02]  euk.gff.mod
    ├── [ 30K Apr  9 22:13]  fun3tax_10.noidfilter.wranks
    ├── [ 29K Apr  9 22:13]  fun3tax_10.wranks
    ├── [ 32K Apr  9 22:13]  fun3tax_11.noidfilter.wranks
    ├── [ 31K Apr  9 22:13]  fun3tax_11.wranks
    ├── [ 34K Apr  9 22:13]  fun3tax_12.noidfilter.wranks
    ├── [ 33K Apr  9 22:13]  fun3tax_12.wranks
    ├── [ 27K Apr  9 22:13]  fun3tax_13.noidfilter.wranks
    ├── [ 27K Apr  9 22:13]  fun3tax_13.wranks
    ├── [ 30K Apr  9 22:13]  fun3tax_14.noidfilter.wranks
    ├── [ 30K Apr  9 22:13]  fun3tax_14.wranks
    ├── [ 28K Apr  9 22:13]  fun3tax_15.noidfilter.wranks
    ├── [ 27K Apr  9 22:13]  fun3tax_15.wranks
    ├── [ 32K Apr  9 22:13]  fun3tax_16.noidfilter.wranks
    ├── [ 30K Apr  9 22:13]  fun3tax_16.wranks
    ├── [ 28K Apr  9 22:13]  fun3tax_17.noidfilter.wranks
    ├── [ 27K Apr  9 22:13]  fun3tax_17.wranks
    ├── [ 29K Apr  9 22:13]  fun3tax_18.noidfilter.wranks
    ├── [ 28K Apr  9 22:13]  fun3tax_18.wranks
    ├── [ 30K Apr  9 22:13]  fun3tax_19.noidfilter.wranks
    ├── [ 30K Apr  9 22:13]  fun3tax_19.wranks
    ├── [ 32K Apr  9 22:12]  fun3tax_1.noidfilter.wranks
    ├── [ 32K Apr  9 22:12]  fun3tax_1.wranks
    ├── [ 32K Apr  9 22:13]  fun3tax_20.noidfilter.wranks
    ├── [ 31K Apr  9 22:13]  fun3tax_20.wranks
    ├── [ 29K Apr  9 22:13]  fun3tax_21.noidfilter.wranks
    ├── [ 29K Apr  9 22:13]  fun3tax_21.wranks
    ├── [ 29K Apr  9 22:13]  fun3tax_22.noidfilter.wranks
    ├── [ 28K Apr  9 22:13]  fun3tax_22.wranks
    ├── [ 29K Apr  9 22:13]  fun3tax_23.noidfilter.wranks
    ├── [ 29K Apr  9 22:13]  fun3tax_23.wranks
    ├── [ 28K Apr  9 22:13]  fun3tax_24.noidfilter.wranks
    ├── [ 27K Apr  9 22:13]  fun3tax_24.wranks
    ├── [ 29K Apr  9 22:13]  fun3tax_25.noidfilter.wranks
    ├── [ 29K Apr  9 22:13]  fun3tax_25.wranks
    ├── [ 31K Apr  9 22:13]  fun3tax_26.noidfilter.wranks
    ├── [ 30K Apr  9 22:13]  fun3tax_26.wranks
    ├── [ 29K Apr  9 22:13]  fun3tax_27.noidfilter.wranks
    ├── [ 28K Apr  9 22:13]  fun3tax_27.wranks
    ├── [ 28K Apr  9 22:13]  fun3tax_28.noidfilter.wranks
    ├── [ 27K Apr  9 22:13]  fun3tax_28.wranks
    ├── [ 30K Apr  9 22:14]  fun3tax_29.noidfilter.wranks
    ├── [ 30K Apr  9 22:14]  fun3tax_29.wranks
    ├── [ 29K Apr  9 22:12]  fun3tax_2.noidfilter.wranks
    ├── [ 28K Apr  9 22:12]  fun3tax_2.wranks
    ├── [ 27K Apr  9 22:14]  fun3tax_30.noidfilter.wranks
    ├── [ 27K Apr  9 22:14]  fun3tax_30.wranks
    ├── [ 29K Apr  9 22:14]  fun3tax_31.noidfilter.wranks
    ├── [ 28K Apr  9 22:14]  fun3tax_31.wranks
    ├── [ 28K Apr  9 22:14]  fun3tax_32.noidfilter.wranks
    ├── [ 27K Apr  9 22:14]  fun3tax_32.wranks
    ├── [ 32K Apr  9 22:12]  fun3tax_3.noidfilter.wranks
    ├── [ 32K Apr  9 22:12]  fun3tax_3.wranks
    ├── [ 30K Apr  9 22:12]  fun3tax_4.noidfilter.wranks
    ├── [ 30K Apr  9 22:12]  fun3tax_4.wranks
    ├── [ 32K Apr  9 22:12]  fun3tax_5.noidfilter.wranks
    ├── [ 30K Apr  9 22:12]  fun3tax_5.wranks
    ├── [ 33K Apr  9 22:13]  fun3tax_6.noidfilter.wranks
    ├── [ 32K Apr  9 22:13]  fun3tax_6.wranks
    ├── [ 26K Apr  9 22:13]  fun3tax_7.noidfilter.wranks
    ├── [ 26K Apr  9 22:13]  fun3tax_7.wranks
    ├── [ 27K Apr  9 22:13]  fun3tax_8.noidfilter.wranks
    ├── [ 27K Apr  9 22:13]  fun3tax_8.wranks
    ├── [ 33K Apr  9 22:13]  fun3tax_9.noidfilter.wranks
    ├── [ 32K Apr  9 22:13]  fun3tax_9.wranks
    ├── [  16 Apr  9 21:02]  mito.gff
    ├── [  16 Apr  9 21:02]  mito.gff.mod
    ├── [6.9K Apr  9 21:02]  trna.gff.mod
    ├── [ 75K Apr  9 21:02]  trnas.aragorn
    └── [  89 Apr  9 22:12]  wc

8 directories, 113 files

Also, I have checked /hdd/mojf/output/B11_Eukaryote_TEST/results/06.B11_Eukaryote_TEST.fun3.tax.wranks and /hdd/mojf/output/B11_Eukaryote_TEST/results/06.B11_Eukaryote_TEST.fun3.tax.noidfilter.wranks.

The first 10 lines in 06.B11_Eukaryote_TEST.fun3.tax.wranks were as follows.

The first 10 lines in 06.B11_Eukaryote_TEST.fun3.tax.noidfilter.wranks were as follows.

I think this may be an error caused by incorrect content in the first column of these two files. But I don't know what should I do to correct this error. Or is there possible other error?

Looking forward to your solution. I will be very grateful for your help.

[jtamames]

Hello
Sorry for my very late renspose to this one, somehow I overlooked this issue.
As I as saying above:

you could replace the 03.faa (aa sequences), 03.fna (nucleotide sequences) and 03.gff (information for ORFs) file for new ones you have from funannotate (keeping the ORF naming schema of SqueezeMeta, which is contigname_ORFinitpos_ORFendpos).

But your files still have the original funannotate format, something like FUN00001_1-T1. The right format could be something like FUN00001_1_128, being 1 and 128 the starting and end positions of the ORF in the contig. Notice that in this case, the contig name will be FUN00001, so you would need to change the gff accordingly.

Best,
J