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I was wondering if anyone had the opportunity to test parameters for computing ANI between viral genomes.
I guess that the defaults of fragment length of 3000 and minimal number of fragments of 50 is too conservative for small genomes.
The text was updated successfully, but these errors were encountered:
I too have this question. When i tried running 2 exact same viral contig fna files, the output is none, indicating an ANI<80%, which obviously is incorrect.
It might be because you have less fragments than the minimal number of fragments cutoff.
The latest version of fastANI use minimal fraction of fragments instead (and I also recommend to reduce the fragments length a bit)
It might be because you have less fragments than the minimal number of fragments cutoff. The latest version of fastANI use minimal fraction of fragments instead (and I also recommend to reduce the fragments length a bit)
I was wondering if anyone had the opportunity to test parameters for computing ANI between viral genomes.
I guess that the defaults of fragment length of 3000 and minimal number of fragments of 50 is too conservative for small genomes.
The text was updated successfully, but these errors were encountered: